Field Notes Journal a personal natural history of place

VI.4 Examination of Microbial Films

Aim

To observe the structure of microbial communities forming surface films.

Specimen & Apparatus

• Surface film from standing water
• Pipette or dropper
• Slide and coverslip
• Compound microscope

Method

1. Collect a small sample of the surface film using a pipette.
2. Place a drop on a slide.
3. Apply a coverslip carefully.
4. Examine under low power to observe overall structure.
5. Increase magnification to study finer detail.

Observations to Make

• Structure and consistency of the film.
• Presence of different types of organisms.
• Arrangement of material within the film.
• Any visible movement or activity.