Field Notes Journal a personal natural history of place

VI.2 Examination of Decaying Leaf Tissue

Aim

To observe structural changes in plant tissue during decay.

Specimen & Apparatus

• Decaying leaf material
• Forceps or needle
• Slide and coverslip
• Water
• Compound microscope

Method

1. Select a small portion of softened leaf tissue.
2. Place on a slide with a drop of water.
3. Gently tease apart the material if required.
4. Apply a coverslip carefully.
5. Examine under low and then higher magnification.

Observations to Make

• Breakdown of cell structure.
• Presence of microorganisms or fungal growth.
• Changes in colour or texture of tissue.
• Differences between intact and degraded areas.