V.4 Observation of the Shoot Apex

Aim

To observe the structure of the growing tip of a plant shoot.

Specimen & Apparatus

• Young shoot tip (e.g. ivy or bean seedling)
• Sharp blade or needle
• Slide and coverslip
• Water
• Compound microscope

Method

1. Remove the tip of a young shoot.
2. Prepare a thin longitudinal section if possible, or examine a small portion directly.
3. Place on a slide with a drop of water.
4. Apply a coverslip carefully.
5. Examine under low power, then increase magnification.

Observations to Make

• Shape and organisation of the shoot tip.
• Presence of very small, closely packed cells.
• Arrangement of developing structures around the apex.
• Differences between the tip and more mature regions.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.