V.3 Examination of a Developing Leaf

Aim

To study the structure of a leaf during early development.

Specimen & Apparatus

• Young leaf from an opening bud (e.g. sycamore or rose)
• Forceps or needle
• Slide and coverslip
• Water
• Compound microscope

Method

1. Remove a young, partially expanded leaf from a bud.
2. Place a small portion on a slide with a drop of water.
3. Apply a coverslip carefully.
4. Examine under low power to observe overall form.
5. Increase magnification to study finer structure.

Observations to Make

• Degree of leaf expansion and folding.
• Early development of veins.
• Shape and arrangement of cells.
• Differences between developing and mature tissue (if known).

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.