V.2 Section of a Dormant Bud

Aim

To examine the internal structure of a dormant bud.

Specimen & Apparatus

• Bud (e.g. ivy or hawthorn)
• Sharp blade or razor
• Slide and coverslip
• Water
• Compound microscope

Method

1. Cut the bud longitudinally using a sharp blade.
2. Select a thin section from the cut surface.
3. Place the section on a slide in a drop of water.
4. Apply a coverslip carefully.
5. Examine under low power, then increase magnification.

Observations to Make

• Arrangement of internal structures within the bud.
• Presence of folded or undeveloped leaves.
• Differences between outer and inner regions.
• Compactness of tissues.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.