V.1 Examination of Bud Scales

Aim

To observe the structure of protective bud scales.

Specimen & Apparatus

• Bud (e.g. horse chestnut or beech)
• Forceps or needle
• Slide and coverslip
• Water
• Compound microscope

Method

1. Remove a bud from the plant.
2. Carefully separate one or more outer scales using forceps.
3. Place the material on a slide with a drop of water.
4. Apply a coverslip gently.
5. Examine under low and then higher magnification.

Observations to Make

• Shape and thickness of bud scales.
• Surface texture and structure.
• Arrangement of layers within the scale.
• Any variation between outer and inner scales.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.