III.5 Examination of Root Structure

Aim

To observe the internal structure of a plant root.

Specimen & Apparatus

• Onion root or young seedling root
• Sharp blade or razor
• Slide and coverslip
• Water
• Compound microscope

Method

1. Cut a thin transverse section of the root.
2. Place the section on a slide in a drop of water.
3. Apply a coverslip carefully.
4. Examine under low power to observe overall structure.
5. Increase magnification to study finer detail.

Observations to Make

• Arrangement of tissues within the root.
• Differences between outer and inner regions.
• Shape and organisation of cells.
• Presence of any central structures.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.