III.2 Cross-Section of a Woody Stem

Aim

To observe the structure of early woody tissue in a young stem.

Specimen & Apparatus

• Young woody twig (e.g. ash, elder, or hawthorn)
• Sharp blade or razor
• Slide and coverslip
• Water
• Compound microscope

Method

1. Cut a thin transverse section of the twig.
2. Place the section on a slide in a drop of water.
3. Apply a coverslip carefully.
4. Examine under low power to observe overall structure.
5. Increase magnification to study finer details.

Observations to Make

• Thickness and structure of outer layers.
• Presence and arrangement of woody tissue.
• Differences between inner and outer regions.
• Any evidence of growth patterns or rings.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.