II.1 Examination of Pollen from Flowering Plants

Aim

To observe the form and surface structure of pollen grains.

Specimen & Apparatus

• Fresh flower (e.g. lily, tulip, or daisy)
• Needle or fine brush
• Slide and coverslip
• Water
• Compound microscope

Method

1. Collect a small quantity of pollen from the anthers using a needle or brush.
2. Transfer the pollen to a slide.
3. Add a small drop of water to disperse the grains.
4. Apply a coverslip carefully.
5. Examine under low power to locate grains, then increase magnification.

Observations to Make

• General shape and size of pollen grains.
• Surface texture or sculpturing.
• Presence of apertures or pores.
• Degree of uniformity among grains.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.