I.2 Observation of Stomata

Aim

To study the structure and distribution of stomata in a leaf epidermis.

Specimen & Apparatus

• Fresh leaf (e.g. spider plant, tradescantia, or lily)
• Forceps or needle
• Slide and coverslip
• Water
• Compound microscope

Method

1. Prepare an epidermal peel from the lower surface of the leaf.
2. Mount the peel in a drop of water on a slide.
3. Apply a coverslip carefully.
4. Focus under low power and locate stomata.
5. Increase magnification for detailed examination.

Observations to Make

• Shape and arrangement of guard cells.
• Form of the stomatal aperture.
• Distribution of stomata across the surface.
• Differences between surrounding epidermal cells and guard cells.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.