I.1 Examination of the Epidermis of a Leaf

Aim

To observe the arrangement and form of epidermal cells in a leaf.

Specimen & Apparatus

• Fresh leaf (e.g. ivy, privet, or laurel)
• Forceps or needle
• Slide and coverslip
• Water
• Compound microscope

Method

1. Remove a small portion of the leaf.
2. Gently peel a thin strip of the epidermis from the surface using forceps.
3. Place the peel in a drop of water on a slide.
4. Apply a coverslip carefully to avoid air bubbles.
5. Examine under low power, then increase magnification.

Observations to Make

• Shape and arrangement of epidermal cells.
• Appearance of cell walls.
• Presence or absence of chloroplasts.
• Regularity or variation in the cellular pattern.

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.