0.5 Observation of Limits of Resolution

Aim

To observe the limits of detail visible under high magnification.

Specimen & Apparatus

• Diatom test slide or fine fabric fibres
• Compound microscope (high power objective)

Method

1. Place the specimen on the stage and focus under low power.
2. Increase to higher magnification and refocus carefully.
3. Adjust illumination and condenser position to optimise clarity.
4. Examine the finest visible structures in the specimen.

Observations to Make

• Smallest distinguishable structures or lines.
• Points at which detail becomes indistinct or merges.
• Effect of illumination on resolving fine detail.
• Differences between specimens (if more than one is used).

⚠️ Safety: Please read the Safety & Disclaimer page before attempting this exercise.